Amusan encountered a false-start scare during the initial race attempt, which could have led to her disqualification.
Nevertheless, the officials decided to go easy on her and issued her a yellow card instead.
With that victory, she secured her third consecutive African Games championship.
Amusan emerged victorious over SidonieFiadanantsoa of Madagascar, who finished in second place with a time of 13.19 seconds.
Faith Osamuyi from Nigeria narrowly missed securing a spot on the podium, finishing in 4th place with a time of 13.77 seconds.
Amusan holds the current world record in the 100m hurdles, clocking an impressive time of 12.12 seconds.