Olympic champions Canada dominated the opening exchanges and scored the decisive goal in the 18th minute.
The Super Falcons had numerous chances to equalise but were let down by their poor finishing.
Paris Saint-Germain’s new signing Jennifer Echegini hit the woodwork seven minutes from time.
The defeat was Super Falcons’ first in 2024.
Randy Waldrum’s side will now shift attention to their opening fixture against Brazil at the 2024 Olympics.
The Group C clash will hold at the Stade Bordeaux next Thursday.