Gloria was born on 4 February 1964 in Ilesa, a city in Osun State, southwestern Nigeria. She was raised a Muslim, but converted to Christianity. She studied English language and religious studies at Oyo State College of Education in Ilesha. She continued her education and received teacher preparation at the Divisional Teachers Training College in Ipetumodu.
She met Mike Bamiloye in 1983 and eventually married him. Together, they co-founded the Mount Zion Faith Ministry on August 5, 1985. She has starred in, directed and produced several Christian Nigerian movies and dramas. In 2002, she wrote a book, The Anxiety of Single Sisters.
Mike and Gloria first met at Oyo State College of Education in Ilesha. On October 8, 1988, they married. Gloria was 24, and Mike was 28. However, because his only employment was his full-time involvement in a theatrical ministry, the marriage went against her parents’ wishes.